Objective To investigate the clinical effect of gemcitabine combined with fludarabine and cyclophosphamide regimen in the treatment of non-Hodgkin lymphoma.Methods A total of 64 non-Hodgkin lymphoma patients were randomly divided into treatment group and control group,each group 32 cases.Control group was treated with gemcitabine 1000 mg · m-2,intravenous infusion for 30 min.Treatment group was given fludarabine 25 mg · m-2,cyclophosphamide 15 mg · m-2 on the basis of control group,intravenous infusion for 30 min.Four weeks a course,all patients were treated for 3 courses.The clinical efficacy,physical condition score,pain degree and adverse drug reaction in two groups were compared.Results After treatment,total effective rate in treatment group was 96.88％ (31/32 cases),had significant difference with that in control group,which was 78.13％ (27/32 cases,P ＜0.05).Kamofsky scores before and after treatment in treatment group were 58.69 ± 4.13,86.88 ± 3.48,with significant difference (P ＜ 0.05).The visual analogue scale (VAS) in treatment group and control group after treatment were 1.44 ± 1.21,4.13-± 1.36,with significant difference (P ＜ 0.05).There were 4 cases of white blood cells and platelets reduced,3 cases of allergy,1 case of nausea in treatment group,the incidence of adverse drug reactions was 25.00％ (8/32 cases).There were 7 cases of white blood cells and platelets reduced,5 cases of allergy,3 cases of nausea in control group,the incidence of adverse drug reactions was 46.88％ (15/32 cases),with no significant difference (P ＜ 0.05).Conclusion The clinical efficacy of gemcitabine combined with fludarabine and cyclophosphamide regimen in the treatment of non-Hodgkin lymphoma is better than single application of gemcitabine.It can not only improve patients' physical condition,but also can relieve the pain.