Laboratory Medicine Online  2012;2(1):55-58

doi:10.3343/lmo.2012.2.1.10

Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae.

Won Young JIN 1 ; Sook Jin JANG ; Young Sook KIM ; Geon PARK ; Dae Soo MOON ; Young Jin PARK

Affiliations

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Keywords

Extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae; MicroScan; Phoenix; Double-disk synergy test

Country

Republic of Korea

Language

Korean

Abstract

The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.