Objective　Study on the spleen volume and serum inflammatory factors in predicting the severity of acute ischemic stroke.Methods　A total of 62 patients with AIS within 24 hours of onset,admitted to our hospital from January 2021 to June 2022,were selected as the study subjects.According to the National Institutes of Health Stroke Scale(NIHSS),they were divided into mild group (NIHSS<6 points，n=30) and moderate as well as severe group (NIHSS≥6 points，n=32).The differences in serum inflammatory factors and normalized difference in spleen volume between the two groups were compared.ROC curve was used to analyze value of serum inflammatory factors and normalized difference in spleen volume in evaluating the severity of AIS.Results　There were statistically significant differences in IL-5,IL-6,IL-1β,IL-8,IL-17,IL-12P70,TNF-α and normalized difference in spleen volume between two groups (P<0.05).The area under ROC curve (AUC) of IL-6,IL-12P70 and normalized difference in spleen volume for assessing AIS severity were 0.715,0.715 and 0.826,respectively.Normalized difference in spleen volume had the largest AUC prediction,and the sensitivity and specificity were 90.0% and 74.1%.Conclusion　Normalized difference in spleen volume and serum inflammatory factors are correlated with the severity of AIS,and can be used as indicators to predict the severity of AIS.