Objective To investigate the relationship between serum visfatin levels and glycometabolism in gestational diabetes mellitus(GDM). Methods 80 cases pregnant women were divided into two groups: GDM group and normal glucose tolerance group(control group). The levels of serum visfatin、fast insulin(FIN)、fast plasma glucose(FPG)、1h、2h、3h plasma glucose after glucose loading(1hPG、2hPG、3hPG )were measured. Insulin resistance index(HOMA-IR) was calculated. Results (1)The levels of visfatin、FPG、1hPG、2hPG、3hPG、HOMA-IR、FIN were significantly higher in GDM group than control group(P＜0.01). (2)There was a positive correlation between visfatin and HOMA-IR and 2hPG(P＜0.01). Multiple regression analysis showed that 2hPG was independent related factor. Conclusion Increased serum levels of visfatin represents a compensatory mechanism aimed at high glucose levels in women with GDM.[Key Words] Visfatin;Diabetes;Gestational;Insulin resistance