Identification and analysis of AP2/ERF gene family of Panax notoginseng and function of PnDREB84 gene
10.16438/j.0513-4870.2023-0203
- VernacularTitle:三七AP2/ERF基因家族鉴定及PnDREB84基因功能初探
- Author:
Shuang-hua ZHANG
1
;
Cong HOU
2
;
Yu-fei CHENG
2
;
Kang NING
2
;
Jun-zhi WANG
3
;
Lin-lin DONG
2
Author Information
1. College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China; Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China
- Publication Type:Research Article
- Keywords:
italic>Panax notoginseng;
AP2/ERF family;
italic>Fusarium oxysporum;
low temperature stress;
italic>PnDREB84 gene
- From:
Acta Pharmaceutica Sinica
2023;58(11):3428-3438
- CountryChina
- Language:Chinese
-
Abstract:
The AP2/ERF gene family is one of the largest transcription factor families in the plant kingdom, and plays an important role in response to biological and abiotic stresses, plant hormone responses, and plant growth and development. In this study, the AP2/ERF family of Panax notoginseng was identified by bioinformatics methods, and the physicochemical properties, structure, phylogenetic relationship, expression pattern and function of PnDREB4 gene of the family were analyzed. The results showed that 140 AP2/ERF family members were identified in P. notoginseng, which were divided into DREB, ERF, AP2, RAV and Sololit subgroups. The physicochemical properties and motifs of proteins were similar among the subgroups. There were 34 differentially expressed genes in the AP2/ERF family of Fusarium oxysporum infected P. notoginseng plants, and 19 genes were up-regulated. The expression level of PnDREB84 was up-regulated with the extension of Fusarium oxysporum infection time in the range of 0-96 h. The content of ABA and SA in P. notoginseng plants overexpressing PnDREB84 gene increased after 4 ℃ stress. The results showed that PnDREB84 gene plays a dual regulatory role in the process of biological stress and abiotic stress. PnDREB84 gene can be used as a potential molecular marker for the breeding of new varieties of P. notoginseng. The identification of AP2/ERF transcription factor and function analysis of PnDREB84 gene of P. notoginseng provided data support for the analysis of stress resistance mechanism of P. notoginseng and the breeding of new varieties.
