Simultaneous determination of fifteen components in Niuhuang Jiangya Pills by UPLC-Q Exactive-Orbitrap MS
10.16438/j.0513-4870.2023-0066
- VernacularTitle:UPLC-Q Exactive-Orbitrap MS法同时测定牛黄降压丸中15种胆酸类成分的含量
- Author:
Qiu-mei HUANG
1
;
Shi-ling LIN
2
;
Ming SHI
1
;
Yan-fang ZHENG
2
;
Ming-qing HUANG
2
Author Information
1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China
2. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
- Publication Type:Research Article
- Keywords:
UPLC-Q Exactive-Orbitrap MS;
Niuhuang Jiangya Pill;
cholic acid;
quantitative analysis
- From:
Acta Pharmaceutica Sinica
2023;58(9):2777-2784
- CountryChina
- Language:Chinese
-
Abstract:
An ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Exactive-Orbitrap MS) method for the simultaneous determination of 15 compounds (taurocholic acid, 7-keto-3α,12-α-dihydroxycholanic acid, glycocholic acid, 3-oxo-7α,12α-hydroxy-5β-cholanoic acid, taurochenodeoxycholic acid, 3α-hydroxy-7-oxo-5β-cholanic acid, hyocholic acid, sodium taurodeoxycholate, hyodeoxycholic acid, cholic acid, glycochenodeoxycholic acid, glycodeoxycholic acid, taurolithocholic acid sodium salt, chenodeoxycholic acid, deoxycholic acid) in Niuhuang Jiangya Pills was established. The separation was performed on a Thermo Fisher Scientfic Bremen HYPERSIL GOLD C18 column (100 mm × 2.1 mm, 1.9 μm). Methanol and water (containing 0.1% formic acid)were adopted as the mobile phase by gradient elution．MS detection was performed with multiple reaction monitoring mode．The results showed that fifteen compounds had a good linearity within their respective concentration ranges (r > 0.999 0). The average recovery rates were 93.7%- 105.2% (n = 9). The established method was used to determine the content of 15 batches of samples, and the results showed that the content of cholic acid was quite different. The present study provides an important reference for the overall quality control of Niuhuang Jiangya Pills.
