Effect of Spirulina on Cochlea Histopathological Changes in Wistar Rats Induced by Kanamicin
https://doi.org/10.47836/mjmhs.19.2.12
- Author:
Prima Erlangga Harinto
1
;
Dian Ayu Ruspita
1
;
Dwi Marliyawati
1
;
Pujo Widodo
1
;
Zulfikar Naftali
1
Author Information
1. Ear Nose Throat Head and Neck Surgery Unit, Department of ENT-HNS, Faculty of Medicine, Diponegoro University, 50275, Semarang, Central Java, Indonesia
- Publication Type:Journal Article
- Keywords:
Histopathology, Kanamycin, Ototoxicity, Spirulina
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.2):69-75
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Streptomycin and kanamycin are aminoglycosides that are toxic to the cochlea vestibular system, can
causing hearing loss. This antibiotic is used for the treatment of tuberculosis and its ototoxicity occurs in 20% of
tuberculosis patients. Spirulina is a cyanobacterial species that is used as a dietary supplement and contains phycocyanin compounds that function as antioxidants and anti-inflammatory. The aim of this study was to determine the
effect of spirulina on histopathological changes in the cochlea in Wistar rats after kanamycin induction. Methods:
this study is a form of posttest-only controlled group design research with a sample of 24 wistar rats divided into
4 groups, namely negative control group, positive control group, treatment group 1 and treatment group 2. Observations of the study took place in November-December 2021. Histopathological measurements in hair cells, macrophages and cochlear vasculature. The analysis used non-parametric Kruskal-Wallis and post-hoc Mann-Whitney
tests. Results: There were more hair cell damage, macrophage cell count, and significant vascular dilatation in the
kanamycin group than in the without kanamycin group with the value p=0.001. There was significantly less number
of hair cell damage in the kanamycin group with spirulina at a dose of 1000 mg than in the kanamycin group with
spirulina at a dose of 400 mg p=0.045. Conclusion: There was a significant effect on the administration of spirulina
on histopathological changes in the cochlea of rats.
- Full text:11.2023my1462.pdf
