Effect of hydrogen on cell pyroptosis during lung injury in severely burned rats
10.3760/cma.j.cn131073.20230301.00722
- VernacularTitle:氢对重度烧伤大鼠肺损伤时细胞焦亡的影响
- Author:
Chao QIN
1
;
Ning LUO
;
Yonghao YU
Author Information
1. 天津医科大学总医院麻醉科 天津市麻醉学研究所，天津 300052
- Keywords:
Hydrogen;
Burns;
Lung;
Pyroptosis
- From:
Chinese Journal of Anesthesiology
2023;43(7):873-876
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of hydrogen on cell pyroptosis during lung injury in severely burned rats.Methods:Forty-eight clean-grade healthy adult male Sprague-Dawley rats, weighing 200-230 g, aged 6-7 weeks, were divided into 4 groups ( n=12 each) by the random number table method: sham operation group (SH group), sham operation+ hydrogen group (SH+ H 2 group), burn group (B group) and burn+ hydrogen group (B+ H 2 group). In group B and group B+ H 2, the Ⅲ degree burn model of the back (about 40% of the total body surface area) was prepared in anesthetized animals. The back was shaved and then subjected to a perm at skin temperature in group SH and group SH+ H 2. Rats were exposed to 2% hydrogen for 1 h starting from 1 and 6 h after burn in SH+ H 2 group and B+ H 2 group, respectively. At 12 and 24 h after burn, the rats were sacrificed and lung tissues were obtained for examination of pathological changes which were scored and for determination of the contents of interleukin-1beta (IL-1β) and IL-18 in lung tissues (by enzyme-linked immunosorbent assay) and expression of NOD-like receptor thermal protein domain associated protein 3 (NLRP3), caspase-1 and Gasdermin D protein (GSDMD) in lung tissues (by Western blot). Results:Compared with SH group, the pathological scores were significantly increased at 12 and 24 h after burn in group B ( P<0.05). The pathological scores were significantly decreased at 12 and 24 h after burn in group B+ H 2 as compared with group B ( P<0.05). Compared with SH group, the contents of IL-1β and IL-18 in lung tissues were significantly increased at 12 and 24 h after burn, and the expression of NLRP3, caspase-1 and GSDMD was up-regulated in B and B+ H 2 groups ( P<0.05). Compared with group B, the contents of IL-1β and IL-18 in lung tissues were significantly decreased at 12 and 24 h after burn, and the expression of NLRP3, caspase-1 and GSDMD was down-regulated in group B+ H 2 ( P<0.05). Conclusions:The mechanism by which hydrogen attenuates lung injury may be related to inhibition of cell pyroptosis in severely burned rats.
