Effects of miR-562 targeting FGFR1 on the migration and invasion of colorectal cancer cells
10.3760/cma.j.cn.115807-20210603-00171
- VernacularTitle:miR-562靶向调控FGFR1对结直肠癌细胞迁移及侵袭的影响
- Author:
Yanbo ZHAO
1
;
Lei LIU
;
Huadong ZHOU
;
Lin LIU
Author Information
1. 十堰市人民医院（湖北医药学院附属人民医院）急危重症中心急诊科，十堰 442000
- Keywords:
microRNA 562;
Fibroblast growth factor receptor 1;
Colorectal cancer;
Migration;
Invasion
- From:
Chinese Journal of Endocrine Surgery
2022;16(6):662-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of microRNA 562 (miR-562) regulating fibroblast growth factor receptor 1 (FGFR1) on the migration and invasion of colorectal cancer cells.Methods:From October 2019 to October 2020, 25 colorectal cancer patients undergoing surgical resection in our hospital were selected to obtain their colorectal cancer tissue and normal adjacent tissue samples at the tumor edge>5 cm; Real-time fluorescent quantitative PCR (qRT-PCR) method was used to detect the expression of miR-562 in adjacent tissues, colon cancer tissues, human normal colorectal cells (FHC cells) and human colorectal cancer cell lines (SW480, SW620, HT29 and HCT116 cells) ; SW480 cells were divided into control group, miR-NC group, miR-562 mimics group, miR-562 mimics+pcDNA3.1 group, and miR-562 mimics+pcDNA3.1-FGFR1 group; The proliferation of SW480 cells was detected by CCK-8 method; Transwell method was used to detect the invasion and migration of SW480 cells; Double luciferase reporter gene was used to detect the targeting relationship between miR-562 and FGFR1; Western blot was used to detect the expression of FGFR1, Cyclin D1 (CyclinD1) and matrix metalloproteinase 2 (MMP2) in SW480 cells.Results:Compared with adjacent tissues, the expression of miR-562 in colon cancer tissues was significantly reduced [ (0.59±0.08) vs (1.01±0.10) ] ( P<0.05) ; compared with FHC cells (1.00±0.08) , the expression level of miR-562 in SW480, SW620, HT29 and HCT116 cells was significantly reduced (0.48±0.06, 0.76±0.14, 0.70±0.11, 0.56±0.10) ( P<0.05) , and its expression level was the lowest in SW480 cells; compared with the control group, the expression level of miR-562 and proliferation inhibition rate in the miR-562 mimics group were significantly increased, the expression levels of FGFR1, CyclinD1, the numbers of migration and invasion cells, and the expression level of MMP2 were significantly reduced ( P<0.05) . FGFR1 was a potential target gene of miR-562. High expression of FGFR1 could reverse the inhibitory effect of miR-562 overexpression on migration and invasion of SW480 cells. Conclusion:The overexpression of miR-562 may inhibit the migration and invasion of SW480 cells by inhibiting the expression of FGFR1.
