Effects of Cistanches Herba polysaccharides on rats with constipation-predominant irritable bowel syndrome
- VernacularTitle:肉苁蓉多糖对便秘型肠易激综合征大鼠的影响
- Author:
Yanzhong LIU
1
;
Xinmei JIA
2
;
Hongzhang GUO
3
;
Xuelian WANG
4
;
Dongsheng LIU
5
Author Information
1. Statistics Advisory Department，Information Center，Gansu Medical Products Administration，Lanzhou 730070，China
2. Dept. of Pharmacy，Wuwei Hospital of Traditional Chinese Medicine，Gansu Wuwei 733000，China
3. Dept. of Gastroenterology，Gansu Provincial Hospital of TCM，Lanzhou 730050，China
4. Drug Inspection Department，Audit and Inspection Center，Gansu Medical Products Administration，Lanzhou 730070，China
5. Laboratory of Chinese Patent Medicine，Gansu Institute for Drug Control，Lanzhou 730070，China
- Publication Type:Journal Article
- Keywords:
Cistanches Herba polysaccharides;
constipation-predominant irritable bowel syndrome;
nerve growth factor/
- From:
China Pharmacy
2023;34(18):2208-2212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of Cistanches Herba polysaccharides （abbreviated as CDPS） on rats with constipation-predominant irritable bowel syndrome （IBS-C）. METHODS SD rats were divided into control group （20 rats） and modeling group. The modeling group was given 2 mL of normal saline at 0-4 ℃ intragastrically （once a day， for 14 consecutive days） to induce IBS-C model. After modeling， model rats were grouped into model group， positive control group （mosapride citrate， 1.35 mg/kg）， CDPS low-dose group （50 mg/kg） and CDPS high-dose group （100 mg/kg）， with 20 rats in each group. Administration groups were given corresponding drug solution intragastrically， and control group and model group were given a constant volume of water intragastrically， once a day， for 28 consecutive days. The fecal water content， serum content of 5-hydroxytryptamine （5-HT）， and charcoal powder propulsion rate of rats were determined in each group；the pathological morphology of colon tissue was observed， and mRNA and protein expressions of nerve growth factor （NGF） and tropomyosin receptor kinase A （TrkA） in colon tissue were detected. RESULTS Compared with control group， the fecal water content and carbon powder propulsion rate in the model group were decreased significantly （P＜0.05）； the rupture of mucosal muscle layer in colon tissue， significant infiltration of inflammatory cells and cellular edema were observed； the content of 5-HT in serum， and relative mRNA and protein expressions of NGF and TrkA were increased significantly （P＜0.05）. Compared with model group， the fecal water content and carbon powder propulsion rate of rats were increased significantly in administration groups （P＜0.05）， and pathological changes of colon tissue were relieved significantly， while the content of 5-HT in serum， the mRNA and protein expressions of NGF and TrkA in colon tissue were decreased significantly （P＜0.05）； among them， the above indicators inthe positive control group and CDPS high-dose group were generally close to those in the control group （P＞0.05）. CONCLUSIONS CDPS can alleviate the symptoms of IBS-C rats， which may be related to the inhibition of NGF/TrkA signaling pathway.
