Determination of 22 phospholipids in serum by ultra performance liquid chromatography-tandem mass spectrometry.
10.3760/cma.j.cn121094-20221021-00508
- VernacularTitle:血清中22种磷脂的超高效液相色谱-串联质谱测定法
- Author:
Wen Rong WANG
1
;
Fang Da PENG
2
;
Tao LI
3
;
Chun Guang DING
2
;
Huan Qiang WANG
3
Author Information
1. National Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China National Key Laboratory for Engineering Control of Dust Hazard, NHC, Beijing 102308, China.
2. National Key Laboratory for Engineering Control of Dust Hazard, NHC, Beijing 102308, China National Center for Occupational Safety and Health, NHC, Beijing 102308, China.
3. National Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
Liquid chromatography-tandem mass spectrometry (LC-MS/MS);
Phospholipids;
Serum
- MeSH:
Humans;
Chromatography, Liquid;
Chromatography, High Pressure Liquid/methods*;
Tandem Mass Spectrometry/methods*;
Phospholipids;
Methanol
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(5):366-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of 22 phospholipids in serum. Methods: In September 2022, Using synthetic non endogenous phospholipids as internal standard, phospholipids in serum were extracted by methanol-dichloromethane (2∶1, V/V) protein precipitation method. Chromatographic separation was achieved on an ACQUITY UPLC BEH shield RP18 column, and the mobile phase was methanol/water (5∶95, V/V) containing 10 mM ammonium formate and methanol. Detection was performed in multiple reaction monitoring mode with ion mode switching. And the method was applied by analyzing phospholipids in the serum of coal workers' pneumoconiosis patients. Results: The 22 phospholipids showed good linear relationships in their respective concentration ranges and the correlation coefficients were higher than 0.990. The spiked recoveries of the 22 phospholipids were 81.03%-121.63% at the three spiked levels. The intra-assay were less than 14.52%, and the inter-assay were less than 15.00%. Conclusion: The method with the advantages of simplicity, stability and high sensitivity, and it can be used for the analysis of phospholipids in serum.
