Metabolomics Changes of Serum in Rats Dying from Untypical Electric Injury.
10.12116/j.issn.1004-5619.2019.06.001
- Author:
Lin Feng LI
1
;
He Wen DONG
2
;
Dan Feng LIU
1
;
Tian TIAN
1
;
Hao DAI
1
;
Min LIU
1
Author Information
1. West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China.
2. Shanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
metabonomics;
electric injuries;
nuclear magnetic resonance spectroscopy;
serum;
rats
- MeSH:
Animals;
Autopsy;
Electric Injuries/blood*;
Magnetic Resonance Spectroscopy;
Metabolome;
Metabolomics;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Forensic Medicine
2019;35(6):645-650
- CountryChina
- Language:English
-
Abstract:
Objective To study the differential metabolites of serum in rats dying from untypical electric injury by 1H nuclear magnetic resonance （1 NMR）-based metabolomics methods, in order to provide clues for identification of death from antemortem untypical electric injury and instant postmortem electric injury. Methods Models of rats dying from untypical electric injury, instant postmortem electric injury, mechanical asphyxia, mechanical injury, and high temperature injury were established. The rats in control group were executed without any treatment. The serums of rats from every group were detected by 1H NMR-based metabolomics technology to screen differential metabolites. Results The rats dying from untypical electric injury group was compared with those from mechanical asphyxia group, mechanical injury group, high temperature injury group, and control group, respectively. Four chemical shift points with diagnostic value, and their corresponding metabolites were screened. These chemical shift points contained many small molecules, such as alcohols, phenols, sugars, amino acids, etc. The death from untypical electric injury group was compared with those from instant postmortem electric injury group and control group, and then eight chemical shift points with diagnostic value and their corresponding metabolites were screened. These chemical shift points contained small molecules, such as sugars, amino acids, esters, nucleic acids, etc. Conclusion The 1H NMR-based metabolomics technology can identify differential metabolites of serum in rats dying from untypical electric injury, therefore it may provide a basis for the diagnosis of death from untypical electric injury and the identification of antemortem electric injury and instant postmortem electric injury.
