SNP in differentially methylated region upstream of H19 gene in Chinese Korean nationality.
- Author:
Wen-tao WEI
1
,
2
;
Xin WANG
3
;
Dong-mei WANG
3
;
Lu-yuan TIAN
3
;
Bao-jie WANG
3
;
Hao PANG
3
;
Mei DING
3
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China. weiwentao0311@
2. com
3. School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Journal Article
- MeSH:
Asian People/genetics*;
China;
DNA/genetics*;
DNA Methylation;
DNA Primers;
Forensic Genetics/methods*;
Gene Frequency;
Genotype;
Haplotypes;
Humans;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide;
RNA, Long Noncoding/genetics*;
Republic of Korea/ethnology*;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2013;29(5):360-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate SNP and distribution of haplotypes in differentially methylated region (DMR) upstream of H19 gene in Chinese Korean nationality in order to provide basic data for forensic application and population genetics research.
METHODS:One hundred and one blood samples from unrelated Chinese Korean individuals and 14 blood samples from 5 Chinese Korean intergenerational families which known genetic relationship were collected. The SNP in DMR upstream of H19 gene were investigated by PCR-cycle sequencing and McrBC digestion followed by PCR. The haplotypes detected by parentally imprinted allele (PIA) method and relevant genetic parameters were calculated.
RESULTS:Thirteen SNPs (rs10840167, rs2525883, rs12417375, rs4930101, rs2525882, rs2735970, rs2735971, rs11042170, rs2735972, rs10732516, rs2071094, rs2107425, and rs4930098) and five haplotypes were detected in 1 174 bp target product in DMR upstream of H19 gene, with 9 SNPs having high discrimination power as good genetic markers. The average gene diversity (GD) of haplotypes was 0.714. The maternal haplotype was confirmed correctly by PIA method from McrBC-digested products of genomic DNA.
CONCLUSION:High polymorphisms exist in DMR upstream of H19 gene in Chinese Korean nationality. And determination of the maternal haplotype could furthermore enhance the forensic identification efficiency of imprinted gene.
