Homer protein expression after focal brain contusion in rats.
- Author:
Fu-Yuan WANG
1
,
2
;
Ru-Bo LI
;
Hong-Xia LIANG
;
Yu SHEN
;
Xiao-Chong GUO
;
Zheng-Yin WANG
Author Information
1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China. wfy021021@
2. com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Blotting, Western;
Brain/pathology*;
Brain Injuries/pathology*;
Carrier Proteins/metabolism*;
Contusions/pathology*;
Disease Models, Animal;
Forensic Pathology;
Homer Scaffolding Proteins;
Immunohistochemistry;
Male;
Random Allocation;
Rats;
Rats, Wistar;
Staining and Labeling;
Time Factors
- From:
Journal of Forensic Medicine
2011;27(6):409-412
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate Homer protein expression after focal brain contusion and explore the relationship between expression and injury time.
METHODS:Focal brain contusion in rats was established and Homer protein expression in brain at different injury intervals after contusion was detected by immunohistochemistry and Western blotting.
RESULTS:A small amount of Homer positive expression cells were detected in control group, sham operated group and experimental group (0.5 h after contusion). The amount of Homer positive expression cells increased after 3 h and reached peak 12 h after contusion. The amount of positive cells continued to decrease 1 d after contusion and to the base level 7 d after contusion. Homer protein expression based on immunohistochemistry and Western blotting had statistical difference among adjacent groups.
CONCLUSION:Expression of Homer protein near the focal contusion area shows time dependence after brain contusion in rats.
