SNP typing of formalin-fixed tissue in individual identification.
- Author:
Yuan LIN
1
;
Li LI
;
Yan LIU
;
Zhen-min ZHAO
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. liny@ssfjd.cn
- Publication Type:Review
- MeSH:
Chromosomes, Human, X;
DNA/isolation & purification*;
DNA Primers;
Female;
Forensic Genetics;
Formaldehyde;
Genotype;
Humans;
Intestines;
Multiplex Polymerase Chain Reaction;
Paraffin Embedding;
Polymorphism, Single Nucleotide;
Sensitivity and Specificity;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Tissue Fixation/methods*
- From:
Journal of Forensic Medicine
2010;26(6):446-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.
METHODS:Genomic DNA was prepared from formalin-fixed tissue. In the event that typing using Sinofiler and MiniFiler kits had failed, 51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). RESULTS Full profiles of X-SNP loci were obtained from the formalin-fixed tissue, while the STR and miniSTR genotyping failed.
CONCLUSION:SNP genotyping technique can be used to obtain more information for formalin fixed tissues.
