Knock-down of long intergenic noncoding RNA cyclooxygenase 2 (lincRNA-COX2) inhibits apoptosis and polarization into M1 in Listeria monocytogenes-infected macrophages.
- Author:
Yurong ZHU
1
;
Shuang HUANG
2
;
Lin LIN
2
;
Fengyuan ZHANG
2
;
Xugan JIANG
2
;
Shengxia CHEN
3
Author Information
1. School of Medicine, Jiangsu University, Zhenjiang 212013; Clinical Microbiology, Linfen Central Hospital, Linfen 041000, China.
2. School of Medicine, Jiangsu University, Zhenjiang 212013, China.
3. School of Medicine, Jiangsu University, Zhenjiang 212013, China. *Corresponding author, E-mail: chensxia@ujs.edu.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis/genetics*;
bcl-2-Associated X Protein/metabolism*;
Caspase 3/metabolism*;
Cyclooxygenase 2/metabolism*;
Listeria monocytogenes/pathogenicity*;
Macrophages/microbiology*;
RNA, Long Noncoding/metabolism*;
RNA, Small Interfering/genetics*;
Animals;
Mice
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(4):289-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of long intergenic non-coding RNA COX2 (lincRNA-COX2) on apoptosis and polarization of Listeria monocytogenes (Lm)-infected RAW264.7 cells. Methods RAW264.7 cells were cultured and divided into control group (uninfected cells), Lm infection group, negative control of small interfering RNA (si-NC) group, si-NC and Lm infection group, small interfering RNA of lincRNA-COX2 (si-lincRNA-COX2) group, si-lincRNA-COX2 and Lm infection group. RAW264.7 cells were infected with MOI=10 Lm for 6 hours, and then the inhibition efficiency of siRNA transfection was detected by fluorescence microscope and quantitative real-time PCR (qRT-PCR). The expression levels of cleaved-caspase-3(c-caspase-3), caspase-3, B-cell lymphoma-2 (Bcl2), Bcl2 associated X protein (BAX), arginase 1 (Arg1), inducible nitric oxide synthase (iNOS) were detected by Western blot analysis. Results c-caspase-3/caspase-3, BAX/Bcl2 and iNOS were significantly up-regulated, while the level of Arg1 was down-regulated in Lm-infected RAW264.7 cells compared with control group. LincRNA-COX2 knockdown inhibited the increase of protein levels for BAX/Bcl2, c-caspase-3/caspase-3 and iNOS in Lm-infected RAW264.7 cells, while the level of Arg1 in Lm-infected RAW264.7 cells was up-regulated. Conclusion Knockdown of lincRNA-COX2 can inhibit cell apoptosis and suppress the macrophage polarization into M1 type in Lm-infected RAW264.7 cells.
