Pharmacokinetic study of five ingredients from Chitong xiaoyanling granules in rats
- VernacularTitle:齿痛消炎灵颗粒中5种成分在大鼠体内的药动学研究
- Author:
Zijian ZHANG
1
;
Wanwan ZHANG
2
;
Jieru ZHANG
2
;
Weiran WANG
2
;
Weiman ZHANG
2
;
Chunying WANG
2
Author Information
1. Dept. of Stomatology，Hebei Provincial TCM Hospital，Shijiazhuang 050011，China
2. Dept. of Pharmaceutical Analysis，School of Pharmacy，Hebei Medical University，Shijiazhuang 050017，China
- Publication Type:Journal Article
- Keywords:
Chitong xiaoyanling granules;
rat;
pharmacokinetics;
LC-MS/MS
- From:
China Pharmacy
2023;34(15):1815-1820
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the pharmacokinetics of paeoniflorin， hesperidin， naringenin， formononetin and enoxolone from Chitong xiaoyanling granules in rats. METHODS Six male SD rats fasted but not deprived of water for 12 h were given Chitong xiaoyanling granules （5.0 g/kg） intragastrically at one time. Blood was collected from inner canthus of rats at different time points after administration. Plasma samples were pretreated with acetonitrile precipitated protein （sulfamethoxazole as internal standard）， and the concentrations of 5 ingredients in plasma were determined by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. The pharmacokinetic parameters of each ingredient were calculated using DAS 3.0 software. RESULTS After intragastric administration of Chitong xiaoyanling granules， tmax and t1/2 of formononetin were the shortest among 5 ingredients （0.25 h and 0.39 h， respectively）. For naringenin and enoxolone， the tmax was shorter， but t1/2 was longer， and there was an obvious double-peak phenomenon in the drug-time curve of naringenin. Compared with the other three components， cmax and AUC of paeoniflorin and hesperidin were not directly proportional to the content of in vitro components. CONCLUSIONS Formononetin， naringin and enoxolone in Chitong xiaoyanling granules were absorbed rapidly in rats， while paeoniflorin and hesperidin were absorbed slowly.
