Construction of cell factories for production of valencene in Saccharomyces cerevisiae
10.16438/j.0513-4870.2022-1382
- VernacularTitle:创建酿酒酵母细胞工厂发酵生产芳香精油瓦伦烯
- Author:
Ting-ting YANG
1
;
Dong WANG
2
;
Wen-hao LI
1
;
Yu-song SHI
2
;
Rong-sheng LI
2
;
Wen-jian MA
3
;
Zhu-bo DAI
2
;
Xue-li ZHANG
2
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
3. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
- Publication Type:Research Article
- Keywords:
synthetic biology;
italic>Saccharomyces cerevisiae;
sesquiterpenoid;
valencene
- From:
Acta Pharmaceutica Sinica
2023;58(6):1619-1628
- CountryChina
- Language:Chinese
-
Abstract:
Valencene, a kind of sesquiterpenoid with a citrus flavor, is mainly found in Valencia orange and is commonly used in cosmetics and food additives, as well as industrial synthetic nootkatone. In this study, synthetic biology was used to create a Saccharomyces cerevisiae cell factory to produce valencene. Fistly, valencene synthase gene (CnVS) from Callitropsis nootkatensis was inserted into the chromosome of the chassis strain YTT-T5. The resulting strain VAL-01 could produce 1.1 mg·L-1 valencene. Protein fusion technique was used, different valencene synthases were compared and the copy number of key genes was adjusted, yielding valencene to 436.4 mg·L-1. Then, knocking-out the transcription factor ROX1 resulted in valencene improvement by 17.4%. Moreover, the induction system of galactose was regulated, transcription factor PDR3 and INO2 were overexpressed. The engineered strain VAL-10 could produce 2 798.6 mg·L-1 valencene by high cell density fermentation method (nearly 2 500 times higher than VAL-01). This study provides a basis for green production of valencene.
