Simultaneously quantitative analysis of 35 components in gualoupi injection using hydrophilic interaction liquid chromatography tandem mass spectrometry
10.16438/j.0513-4870.2022-1123
- VernacularTitle:亲水作用色谱-串联质谱法同时测定瓜蒌皮注射液中35种化合物
- Author:
Xiang TAO
1
;
Jing-xian ZHANG
2
;
Qing HU
2
;
Jian SUN
2
;
Ying DONG
3
;
Jin-guo DING
3
;
Hong YU
2
;
Ying-ying SHEN
2
;
Xiu-hong MAO
2
;
Shen JI
1
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Institute for Food and Drug Control, Shanghai 201203, China
2. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
3. SPH No.1 Biochemical & Pharmaceutical Co., Ltd., Shanghai 200240, China
- Publication Type:Research Article
- Keywords:
gualoupi injection;
hydrophilic interaction liquid chromatography tandem mass spectrometry;
ynamic multiple reaction monitoring;
multi-component quantification
- From:
Acta Pharmaceutica Sinica
2023;58(5):1293-1300
- CountryChina
- Language:Chinese
-
Abstract:
A hydrophilic interaction chromatography tandem mass spectrometry method was developed for simultaneous quantification of 35 components in gualoupi injection. The analytes were separated with an ACQUITY XBridge Amide column using 20 mmol·L-1 ammonium formate aqueous solution (pH 3.0) as mobile phase A and 20 mmol·L-1 ammonium formate (pH 3.0)∶acetonitrile (1∶9) as mobile phase B for gradient elution. Mass spectrometry with dynamic multiple reaction monitoring and external standard method were used for quantitative analysis. A total of 35 components were determined in 10 batches of gualoupi injection. The results showed that the 35 compounds had a good linear relationship within their respective concentration ranges with the correlation coefficients (R2 > 0.998 0), the recoveries ranged from 76.6% to 118.5%. The results showed that γ-aminobutyric acid, trigonelline, alanine, threonine, homoserine, citrulline, and leucine were abundant in gualoupi injection, while nicotinamide, methylsuccinic acid, cytosine and choline account for a low percentege. The present study provides an important reference for elucidation of the effective material basis and the improvement of quality standard of gualoupi injection.
