Based on the metabolic mechanism of UHPLC-Q-Exactive-MS Hippocampus to delay the aging of Drosophila melanogaster
10.16438/j.0513-4870.2022-1178
- VernacularTitle:基于UHPLC-Q-Exactive-MS大海马延缓果蝇衰老的代谢机制研究
- Author:
Yu-kun WANG
1
;
Tian-hui ZHOU
1
;
Li-juan DUAN
1
;
Ai-rong ZHANG
2
;
Xue-mei QIN
1
;
Xiao-xia GAO
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
2. Shanxi Guangyuyuan Traditional Chinese Medicine Co., Ltd., Taigu 030800, China
- Publication Type:Research Article
- Keywords:
italic>Hippocampus kuda Bleeker;
processed with butter;
italic>Drosophila melanogaster;
anti-aging;
metabolomics
- From:
Acta Pharmaceutica Sinica
2023;58(7):1812-1821
- CountryChina
- Language:Chinese
-
Abstract:
Based on the UHPLC-Q-Exactive-MS metabonomics technology, the effect of Hippocampus kuda Bleeker on the life span of Drosophila melanogaster was studied, and the change rule of endogenous metabolites in the aging process of Drosophila melanogaster after the intervention of Hippocampus kuda Bleeker japonicus was explored to clarify the anti-aging mechanism of Hippocampus. The natural aging model of Drosophila melanogaster was used. Different doses of raw Hippocampus and fried Hippocampus were given to observe the effects on the life span, climbing ability, sexual activity, and antioxidant enzyme activity of Drosophila melanogaster. Based on UHPLC-Q-Exactive-MS metabolomics technology, the metabolic profile of the aging Drosophila melanogaster was analyzed using metabonomics technology to explore the mechanism of Hippocampus kuda Bleeker delaying the aging of Drosophila melanogaster. The results showed that raw Hippocampus and crispy Hippocampus (1, 4 mg·mL-1) could significantly prolong the average life span, median life span and maximum life span of male fruit flies, and significantly improve the climbing ability and sexual vitality of fruit flies. Catalase (CAT) and aldehyde content were increased, while malonaldehyde (MDA) content was decreased. Through metabonomics technology, it was identified that the Hippocampus can significantly recall 16 metabolites and participate in the biosynthesis of phenylalanine, tyrosine and tryptophan, starch and sucrose metabolism, tyrosine metabolism, cysteine and methionine metabolism, and histidine metabolism. The anti-aging mechanism is related to amino acid metabolism and sugar metabolism, which provides a substantial scientific basis for the development and utilization of Hippocampus and clarifying its role in senile diseases. The animal experiment of this study was approved by the Ethics Committee of Shanxi University (approval number: SXULL2021028).
