Role of negative regulators on the TLR7 ligand/IFN-γ signaling in the endothelial cells

Baasansuren E; Javkhlan B; Baljinnyam T; Khulan U; Batkhishig M; Enkhsaikhan L; Ulziisaikhan J; Khongorzul B; Baigalmaa B; Galindev B; Tsevelmaa N; Sodnomtsogt L; Nyambayar D; Munkhtuvshin N; Munkhbat B; Bilegtsaikhan Ts

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Role of negative regulators on the TLR7 ligand/IFN-γ signaling in the endothelial cells

VernacularTitle:TLR7-ийн лиганд болон IFN-γ-ийн дохио дамжилтын хоорондын харилцан үйлчлэлд сөрөг зохицуулагч молекулын нөлөөг тодорхойлох нь
Author: Baasansuren E ¹ ; Javkhlan B ¹ ; Baljinnyam T ² ; Khulan U ³ ; Batkhishig M ³ ; Enkhsaikhan L ³ ; Ulziisaikhan J ³ ; Khongorzul B ⁴ ; Baigalmaa B ⁴ ; Galindev B ⁴ ; Tsevelmaa N ⁴ ; Sodnomtsogt L ⁴ ; Nyambayar D ⁴ ; Munkhtuvshin N ⁵ ; Munkhbat B ⁴ ; Bilegtsaikhan Ts ⁴
Author Information

1. Core Laboratory, Science Technology Center, MNUMS;Department of Microbiology and Immunology, SBS, MNUMS
2. Department of Microbiology and Immunology, SBS, MNUMS;Central Scientific Research Laboratory, Institute of Medical Sciences, Mongolia
3. Department of Microbiology and Immunology, SBS, MNUMS
4. Core Laboratory, Science Technology Center, MNUMS
5. Central Scientific Research Laboratory, Institute of Medical Sciences, Mongolia
Publication Type:Journal Article
From: Health Laboratory 2018;8(1):14-18
CountryMongolia
Language:Mongolian
Abstract: Introduction:Toll like receptors (TLRs) are a class of proteins that key role in the innate immune system. The SOCS1 and SHP2 proteins are negative-feed loop inhibitors of signaling of JAK/STAT and TLRs pathways.
Purpose:To determine negative regulator protein activation which is activated through TLR7 ligand/IFN-γ signal transduction in endothelial cells.
Methods:We used mouse aortic linear endothelial cell (END-D); protein expressio was detected by western blotting
Results:We analyzed a time dependent stimulation effects of negative regulator proteins stimulated by TLR7 ligand/IFN-γ in endothelial cell cultures. Imiquimod of 10 μg/ml treatment of 1 hr was followed by 100 ng/ml IFN-γ stimulation for 1-8hr to analysis of negative regulator SOCS1 and SHP2 protein expression.
In untreated cells, there was low activations of negative regulator SOCS1 and SHP2 proteins. IFN-γ stimulation alone had increased SOCS1 and SHP2 protein expressions, also imiquimod treatment highly elevated SOCS1 and SHP2 expressions. However imiquimod and IFN-γ doubled treatment have decreased activation of negative regulator SOCS1 and SHP2 proteins. These findings suggest SOCS1 and SHP2 proteins are inhibitors in the TLR7 ligand/IFN-γ signaling.
Conclusion:Negative regulators, SOCS1 and SHP2 strongly suppressed activations of TLR7 ligand/IFN-γ signaling
Full text:HL-2018-8(2)-14-18.pdf