DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes

Jean NYEMB; Joseph MBAFOR; Jean NYEMB; Roland NDOUBALEM; Emmanuel TALLA; Alembert TCHINDA; Dieudonné NDJONKA; Celine HENOUMONT; Sophie LAURENT

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DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes

Author: Jean NYEMB ¹ ; Joseph MBAFOR ¹ ; Jean NYEMB ² ; Roland NDOUBALEM ² ; Emmanuel TALLA ² ; Alembert TCHINDA ³ ; Dieudonné NDJONKA ⁴ ; Celine HENOUMONT ⁵ ; Sophie LAURENT ⁵
Author Information

1. Department of Organic Chemistry, Faculty of Science, University of Yaounde i
2. Department of Chemistry, Faculty of Science, University of Ngaoundere
3. Institute of Medical Research and Medicinal Plants Studies (IMPM), Ministry of Scientific Research and Innovation
4. Department of Biology, Faculty of Science, University of Ngaoundere
5. Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory
Publication Type:Journal Article
Keywords: Antioxidant; Bergenin; Cissus populnea; Onchocerca ochengi; Phenolic compounds; Phytochemical constituent
From: Asian Pacific Journal of Tropical Medicine 2018;11(4):280-284
CountryChina
Language:Chinese
Abstract: Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin-Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic.