Effect of Recombinant Human Thrombopoietin (rhTPO) on Hematopoietic Reconstitution in Allogeneic Hematopoietic Stem Cell Transplantation Model.
10.19746/j.cnki.issn.1009-2137.2023.01.038
- Author:
Jin QIU
1
;
Li-Ying HAN
2
;
Hong-Yun XING
3
;
Kun-Li GAO
4
;
Tie-Rong BIAN
5
Author Information
1. Department of General Medicine, Luzhou People's Hospital, Luzhou 646000, Sichuan Province, China.
2. Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
3. Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.E-mail: xinghy52003@foxmail.com.
4. Department of General Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
5. Medical Experiment Center, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
- Publication Type:Journal Article
- Keywords:
allogeneic hematopoietic stem cell transplantation;
hematopoietic reconstitution;
mice;
recombinant human thrombopoietin
- MeSH:
Humans;
Animals;
Mice;
Thrombopoietin;
Mice, Inbred C57BL;
Hematopoietic Stem Cell Transplantation;
Hematopoietic Stem Cells;
Bone Marrow;
Recombinant Proteins;
Mice, Inbred BALB C
- From:
Journal of Experimental Hematology
2023;31(1):241-246
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of recombinant human thrombopoietin (rhTPO) on hematopoietic reconstruction in allogeneic hematopoietic stem cell transplantation (allo-HSCT) model.
METHODS:The C57BL/6 mice were employed as the donors, and BALB/c mice as recipients. The bone marrow mononuclear cells of the donor mice were extracted and pretreated, which then were injected with 5×106 per mouse through the tail vein of the recipient to establish an allo-HSCT model. The implantation of hematopoietic stem cells in the recipient mice was detected by flow cytometry on the 28th day after transplantation. Next, the successfully modeled recipient mice were randomly divided into experimental group and control group. The rhTPO was injected into mice in the experimental group on the first day after transplantation, while the saline was injected into mice in the control group. Both groups were injected for 14 consecutive days. The peripheral blood and bone marrow hematopoiesis of the two groups were observed on day 1, 3, 7, 14, and 21 after transplantation.
RESULTS:The expression rate of H-2Kb in the bone marrow of recipient mice was 43.85% (>20%) on the 28th day after transplantation, which indicated that the recipient mice were successfully chimerized. Meanwhile, counts of PLTs on the day 3, 7, 14, and 21 after transplantation in the experimental group were higher than those in the control group with statistical significances (P<0.05). In addition, hematopoietic function of bone marrow was suppressed in both groups on day 1, 3 and 7 after transplantation, but hematopoietic bone marrow hyperplasia was better in the experimental group than in the control group. On day 14 and 21 after transplantation, the hematopoietic function of bone marrow in the two groups was recovered, and the experimental group showed more obvious than the control group.
CONCLUSION:rhTPO can effectively stimulate the production of PLTs and facilitate the recovery of white blood cells and hemoglobin after allo-HSCT, and promote hematopoietic recovery and reconstitution of bone marrow.
