Monoterpenoids from Paeoniae Radix Rubra based on UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20220728.201
- Author:
Xiao HE
1
;
Hui-Min WU
1
;
Le-le XIONG
1
;
Hong-Li JIN
2
;
Yan-Fang LIU
2
;
Ai-Jin SHEN
2
;
Xin-Miao LIANG
2
Author Information
1. Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine, Ganjiang Chinese Medicine Innovation Center Nanchang 330000, China.
2. Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine, Ganjiang Chinese Medicine Innovation Center Nanchang 330000, China Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian 116023, China.
- Publication Type:Journal Article
- Keywords:
Paeoniae Radix Rubra;
UPLC-Q-TOF-MS;
fragmentation patterns;
monoterpenoids with "cage-like" pinane skeleton
- MeSH:
Chromatography, Liquid;
Drugs, Chinese Herbal;
Mass Spectrometry;
Monoterpenes
- From:
China Journal of Chinese Materia Medica
2023;48(4):1005-1013
- CountryChina
- Language:Chinese
-
Abstract:
The ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to conduct the qualitative analysis of the monoterpene chemical components from Paeoniae Radix Rubra. Gradient elution was performed on C_(18) HD(2.1 mm×100 mm, 2.5 μm) column with a mobile phase of 0.1% formic acid(A) and acetonitrile(B). The flow rate was 0.4 mL·min~(-1) and the column temperature was 30 ℃. MS analysis was conducted in both positive and negative ionization modes using electrospray ionization(ESI) source. Qualitative Analysis 10.0 was used for data processing. The identification of chemical components was realized by the combination of standard compounds, fragmentation patterns, and mass spectra data reported in the literature. Forty-one monoterpenoids in Paeoniae Radix Rubra extract were identified. Among them, 8 compounds were reported in Paeoniae Radix Rubra for the first time and 1 was presumed to be the new compound 5″-O-methyl-galloylpaeoniflorin or its positional isomer. The method in this study realizes the rapid identification of monoterpenoids from Paeoniae Radix Rubra and provides a material and scientific basis for quality control and further study on the pharmaceutical effect of Paeoniae Radix Rubra.
