Comparison of alkaloids in Aconiti Kusnezoffii Radix, Aconiti Radix, and Aconiti Lateralis Radix Praeparata based on UHPLC-Q-Exactive Orbitrap MS/MS.
10.19540/j.cnki.cjcmm.20220506.201
- Author:
Sheng-Yun DAI
1
;
Yi-Fang CUI
2
;
Jing XU
2
;
Hong-Yan ZHOU
2
;
Shu-Yi SONG
3
;
Xian-Ming LAN
3
;
Wen-Wen ZHANG
3
;
Jian ZHENG
1
;
Jia-Yu ZHANG
3
Author Information
1. National Institutes for Food and Drug Control Beijing 102629, China.
2. College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250300, China.
3. School of Pharmacy, Binzhou Medical University Yantai 264003, China.
- Publication Type:Journal Article
- Keywords:
Aconiti Kusnezoffii Radix;
Aconiti Lateralis Radix Praeparata;
Aconiti Radix;
UHPLC-Q-Exactive Orbitrap MS/MS;
alkaloids
- MeSH:
Tandem Mass Spectrometry;
Aconitum;
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal;
Alkaloids;
Plants, Medicinal
- From:
China Journal of Chinese Materia Medica
2023;48(1):126-139
- CountryChina
- Language:Chinese
-
Abstract:
UHPLC-Q-Exactive Orbitrap MS/MS was used to systematically analyze and compare the alkaloids in Aconiti Kusnezoffii Radix, Aconiti Radix, and Aconiti Lateralis Radix Praeparata. After the samples were pretreated in the solid-phase extraction cartridges, 0.1% ammonium hydroxide(A)-acetonitrile(B) was used for gradient elution. The LC-MS method for characterization of alkaloids in the three herbal medicines was established in ESI positive ion mode to collect high resolution MS data of reference substances and samples. On the basis of the information of reference substance cracking behavior, retention time, accurate molecular mass, and related literature, a total of 155 alkaloids were identified in Aconiti Kusnezoffii Radix, Aconiti Radix, and Aconiti Lateralis Radix Prae-parata. Specifically, 130, 127, and 92 alkaloids were identified in Aconiti Kusnezoffii Radix, Aconiti Radix, and Aconiti Lateralis Radix Praeparata, respectively. Monoester alkaloids and amino-alcohol alkaloids were dominant in the three herbal medicines, and the alkaloids in Aconiti Kusnezoffii Radix and Aconiti Radix were similar. This paper can provide a reference for elucidating the pharmacological effects and clinical application differences of the three herbal medicines produced from plants of Aconitum.
