Preliminary Results of Single-cell Transcriptome Sequencing in Renal Arterial Lesions of Takayasu Arteritis.
10.3881/j.issn.1000-503X.15006
- Author:
Qing GAO
1
;
Zhi-Yuan WU
2
;
Hai-Yang LI
1
;
Yong-Jun LI
2
Author Information
1. Department of Cardiovascular Surgery,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China.
2. Department of Vascular Surgery,Beijing Hospital,Beijing 100730,China.
- Publication Type:Journal Article
- Keywords:
Takayasu arteritis;
cellular heterogeneity;
endothelial cell;
single-cell RNA sequencing;
smooth muscle cell
- MeSH:
Humans;
Takayasu Arteritis;
Endothelial Cells;
Transcriptome;
Computational Biology;
Fibroblasts
- From:
Acta Academiae Medicinae Sinicae
2023;45(1):80-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preliminary application of single-cell RNA sequencing (scRNA-seq) in the renal arterial lesions in Takayasu arteritis (TA) patients. Methods This study included 2 TA patients with renal artery stenosis treated by bypass surgery in the Department of Vascular Surgery,Beijing Hospital.The obtained 2 renal artery samples were digested with two different protocols (GEXSCOPE kit and self-made digestion liquid) before scRNA-seq and bioinformatics analysis. Results A total of 2920 cells were obtained for further analysis.After unbiased cluster analysis,2 endothelial cell subsets,2 smooth muscle cell subsets,1 fibroblast subset,2 mononuclear macrophage subsets,1 T cell subset,and 1 undefined cell subset were identified.Among them,the two subsets of smooth muscle cells were contractile and secretory,respectively.The results of scRNA-seq indicated that enzymatic hydrolysis with GEXSCOPE kit produced a large number of endothelial cells (57.46%) and a small number of immune cells (13.21%).However,immune cells (34.64%) were dominant in the cells obtained by enzymatic hydrolysis with self-made digestive liquid. Conclusion scRNA-seq can be employed to explore the cellular heterogeneity of diseased vessels in TA patients.Different enzymatic digestion protocols may impact the proportion of different cells.
