Key factors to establish the ovalbumininduced atopic dermatitis minipig model: age and body weight
10.1186/s42826-022-00141-4
- Author:
Young Kyu KIM
1
;
JuKyung LEE
;
Hyeon-Young KIM
;
Sung-Hwan KIM
;
Jeong Ho HWANG
;
Han Na SUH
Author Information
1. Animal Model Research Group, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212 Jeongeup, Jellabuk-do, Republic of Korea
- Publication Type:R E S E A R C H
- From:Laboratory Animal Research
2022;38(4):294-301
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2–4 weeks to induce chronic atopic dermatitis (AD).
Results:Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8–10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response.
Conclusion:Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.
