Preparation and polarization activity research of astragalus polysaccharide-superparamagnetic iron oxide nanocomposite
10.16438/j.0513-4870.2022-1059
- VernacularTitle:黄芪多糖-超顺磁性氧化铁纳米复合物的制备及其诱导巨噬细胞极化的活性研究
- Author:
Lin-qing HUANG
1
;
Xin-meng SHI
1
;
Jing-rong WANG
2
;
Ding QU
1
;
Yu-ping LIU
1
;
Yan CHEN
1
Author Information
1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
2. State Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Macau 999078, China; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
- Publication Type:Research Article
- Keywords:
magnetic iron oxide nanoparticle;
astragalus polysaccharide;
macrophage polarization;
tumor associated macrophage;
nanomedicine
- From:
Acta Pharmaceutica Sinica
2023;58(3):779-788
- CountryChina
- Language:Chinese
-
Abstract:
Size and surface modification are the two key factors affecting the effect of macrophages polarization induced by superparamagnetic iron oxide nanoparticles (SPIONs). The smaller the particle size, the better the polarization effect of SPIONs. Besides, the reasonable SPIONs surface modification method can also be used to enhance the polarization effect. In this study, SPIONs was prepared by solvothermal method and optimized by Box-Benhnken center combination design and response surface method. Furthermore, astragalus polysaccharide-superparamagnetic iron oxide nanocomplex (APS-SPIONs) was successfully constructed by EDC/NHS esterification method. The structure of APS-SPIONs was confirmed by dynamic light scatter and infrared spectrometer, and the contents of iron and polysaccharide were characterized by spectrophotometry. The effect of APS-SPIONs on inducing mouse macrophages RAW264.7 polarization was investigated by flow cytometry. The RAW264.7 macrophages-HepG2 human hepatoma cancer cells Transwell co-culture system was established to investigate APS-SPIONs improve anti-tumor function of macrophages in vitro, and the proliferation activity of APS-SPIONs on RAW264.7 detected by cell counting kit-8 (CCK-8) method. The results showed that the average particle size and zeta potential of APS-SPIONs were (82.93 ± 1.47) nm and (-24.00 ± 0.47) mV. Polysaccharide and Fe content were 8.69% and 7.04%, respectively. APS-SPIONs effectively induced the polarization of RAW264.7 into M1 type in vitro, improving the anti-tumor ability of macrophages in a co-culture system, without effecting the proliferation of macrophages. Our study provides a drug development strategy and preliminary research results to educate macrophages and reshape the tumor immune microenvironment to achieve tumor-killing effects.
