Investigation of metabolites of synthetic cannabinoid 4CN-MDMB-BUTINAC in vitro by liquid-chromatography-quadruple/orbitrap mass spectrometry
10.16438/j.0513-4870.2022-0924
- VernacularTitle:基于UPLC-Q-exactive orbitrap-MS法的新型合成大麻素4CN-MDMB-BUTINACA体外代谢研究
- Author:
Shan-hui ZHOU
1
;
Yu XU
2
;
Zhen-feng YU
1
;
Xing KE
1
;
Yi-lei FAN
1
Author Information
1. Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Zhejiang Police College, Hangzhou 310053, China
2. Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Anti-Drug Laboratory Zhejiang Regional Center, Hangzhou 310053, China
- Publication Type:Research Article
- Keywords:
synthetic cannabinoid;
liver microsome;
metabolite;
metabolic pathway
- From:
Acta Pharmaceutica Sinica
2022;57(12):3653-3659
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the metabolites of a new synthetic cannabinoid 3,3-dimethyl-2-[1-(4-cyanobutyl)indazole-3-formamimino]methyl butyrate (4CN-MDMB-BUTINACA) in vitro, a human liver microsome incubation model was established to analyze the metabolic biotransformation of synthetic cannabinoids using ultra-high performance liquid chromatography coupled to quadrupole-orbitrap high-resolution mass spectrometry. Nontarget metabolomic results showed that the metabolites of 4CN-MDMB-BUTINACA included hydroxylation, ester hydrolysis, ester hydrolysis with hydroxylation reaction, pentane oxidation and ester hydrolysis with pentane oxidation reaction, among which M1-a, M2 and M4 were potential metabolic markers. The research results provide a theoretical basis and technical support for the biomonitoring and metabolic characterisation of the cannabinoid 4CN-MDMB-BUTINACA.
