A pair of new isoflavonoid enantiomers from Dalbergia benthamii Prain
10.16438/j.0513-4870.2022-0768
- VernacularTitle:两粤黄檀中一对新的异黄酮类对映异构体
- Author:
Xiao-yan NING
1
;
Wen-jiao CHEN
1
;
Qing-nan XI
1
;
Xu FENG
1
;
Chen-yan LIANG
2
;
Kai LIU
3
;
Bing LI
1
;
Jian-hua WEI
1
Author Information
1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
2. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China; Guangxi Engineering and Technology Research Center for the Development of Proprietary Chinese Medicine and Ethnic Medicine, Nanning 530200, China
3. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
- Publication Type:Research Article
- Keywords:
italic>Dalbergia benthamii Prain;
isoflavone;
enantiomer;
albergibenthamin;
cytotoxic activity
- From:
Acta Pharmaceutica Sinica
2023;58(1):162-169
- CountryChina
- Language:Chinese
-
Abstract:
Thirteen isoflavones were separated and purified from an ethanol extract of the rhizome of Dalbergia benthamii Prain by using silica gel, Sephadex LH-20, recrystallization et al. Their structures were identified by physicochemical properties and spectral analysis such as MS, 1D/2D-NMR as dalbergibenthamin (1), butesuperin A (2), xanthocercin A (3), butesuperin B (4), di-O-methylalpinum isoflavone (5), 2′-deoxgisoaunculutin (6), robustone (7), 4′-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone (8), formononetin (9), 6″-O-rhamnosyldaidzin (10), 3′,4′-di-O-methylene-5-hydroxy-7-methoxy-6-isopentenyl isoflavone (11), derrubone dimethyl enter (12), and derrubone (13). Compound 1 is a pair of new isoflavonoid enantiomers, compound 12 is a new natural product and compounds 1-7 and 10-13 were obtained from D. benthamii Prain for the first time. In vitro cytotoxic activities of the compounds were explored by MTS testing with HL-60, A-549, SMMC-7721, MCF-7 and SW480 cell lines. Results show that compound 8 significantly inhibited cellular proliferation. The IC50 of compound 8 in A-549 and SW480 cells was 16.68 ± 0.19 and 15.21 ± 0.60 μmol·L-1.
