Quantitative Assessment of Dentine Sialophosphoprotein, Aspartate Aminotransferase and Lactate Dehydrogenase in Gingival Crevicular Fluid of Teeth with Root Resorption
10.21315/aos2022.1702.OA07
- Author:
Noor Ayuni Ahmad Shafiai
1
;
Rohaya Megat Abdul Wahab
2
Author Information
1. Department of Dental Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Kepala Batas, Pulau Pinang, Malaysia
2. Department of Family Oral Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
Aspartate aminotransferase;
Biomarker;
Dentine sialophosphoprotein;
Lactate dehydrogenase;
Root resorption
- MeSH:
Root Resorption;
Gingival Crevicular Fluid;
Dentin;
Aspartate Aminotransferases;
Lactate Dehydrogenases
- From:
Archives of Orofacial Sciences
2022;17(2):237-246
- CountryMalaysia
- Language:English
-
Abstract:
ABSTRACT:Root resorption is a shortening of root dentine which occurs physiologically in deciduous teeth. The
present study aimed to quantify dentine sialophosphoprotein (DSPP), aspartate aminotransferase (AST)
and lactate dehydrogenase (LDH) in gingival crevicular fluid (GCF) during the physiological process
of root resorption of deciduous teeth. A cross-sectional study was conducted with 25 children aged
between 4 and 10 years old. GCF was collected from the gingival sulcus using periopaper strips from the
upper first deciduous molar (n = 45). The samples were divided equally into three groups, no resorption
(R0), moderate resorption (RM) and severe resorption (RS), based on the existing radiographs taken.
The GCF samples were then analysed using an enzyme-linked immunosorbent assay (ELISA) kit
to determine the DSPP concentration levels and BioAssays System kit for AST and LDH. One-way
ANOVA was used to determine the statistical differences between the means of the DSPP, AST and
LDH concentration level in the three groups. A difference was considered significant when p < 0.05.
High concentration levels of DSPP were significantly noted in RS (p < 0.05), compared to RM and
R0. AST also portrayed significant high activity level (p < 0.05) similar to DSPP but LDH showed no
significant changes between groups (p > 0.05). The high quantification of DSPP and AST levels in
the severe and moderately resorbed roots indicated the potential use of this protein as a biomarker for
detecting moderate-severe stages of root resorption.
- Full text:2.2022my0020.pdf
