Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling

Weiwei GUO; Yu DU; Junting GUO; Liping BAI; Qianxiao ZHANG; Yu DU; Junting GUO; Tingting ZHAO; Liping BAI; Xiqiang AN; Qianxiao ZHANG

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Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling

Author: Weiwei GUO ¹ ; Yu DU ¹ ; Junting GUO ¹ ; Liping BAI ¹ ; Qianxiao ZHANG ² ; Yu DU ² ; Junting GUO ² ; Tingting ZHAO ² ; Liping BAI ² ; Xiqiang AN ² ; Qianxiao ZHANG ³
Author Information

1. NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College
2. Key Laboratory for Uighur Medicine, Xinjiang Institute of Materia Medica
3. Department of Chinese Materia Medica, Xinjiang Medical University
Publication Type:Journal Article
Keywords: Akt/NF-κB; bioactivity; Brassica rapa L.; immunomodulatory activity; macrophage; polysaccharide
From: Chinese Herbal Medicines 2022;14(1):90-96
CountryChina
Language:Chinese
Abstract: Objective: To investigate the immunomodulatory activity of polysaccharides from the roots of Brassica rapa. Methods: The crude polysaccharide from roots of B. rapa (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis. Results: Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide (NO), tumor necrosis factor α (TNFα) and upregulating mRNA levels of inducible NO synthase (iNOS) and TNFα. Furthermore, modulation of macrophage by BRP was indicated to be mediated via the activation of Akt and nuclear factor-kappa B (NF-κB). Conclusion: The beneficial effects of BRP could be used as an immunotherapeutic adjuvant in treatment of inflammatory diseases.