Clinical, radiological and molecular diagnosis correlation in serum samples from patients with osteoarticular tuberculosis
10.12980/APJTB.4.201414B112
- Author:
Guadalupe GARCÍA-ELORRIAGA
1
;
César GONZÁLEZ-BONILLA
1
;
Guadalupe GARCÍA-ELORRIAGA
2
;
César GONZÁLEZ-BONILLA
2
;
Olga MARTÍNEZ-ELIZONDO
3
;
Olga MARTÍNEZ-ELIZONDO
4
;
Guillermo DEL REY-PINEDA
5
;
Guillermo DEL REY-PINEDA
6
Author Information
1. Unidad de Investigacion Medica en Inmunologia e Infectología, Hospital de Infectología, Centro Médico Nacional La Raza (CMNR), Instituto Mexicano del Seguro Social (IMSS)
2. Medical Research Unit in Immunology and Infectious Disease, Hospital for Infectious Disease, La Raza National Medical Center (CMNR), Mexican Social Security Institute (IMSS)
3. Hospital de Ortopedia Dr. Victorio de la Fuente Narváez, Instituto Mexicano del Seguro Social (IMSS)
4. Orthopedics Hospital Dr. Victorio de la Fuente Narváez, Mexican Social Security Institute (IMSS)
5. Banco Central de Sangre, CMNR, IMSS, and Departamento de Infectología, Hospital Infantil de México Federico Gómez. Secretaría de Salud (SSA)
6. Central Blood Bank, CMNR, IMSS, Department of Infectious Disease Federico Gomez Children's Hospital. Health Ministry (SSA)
- Publication Type:Journal Article
- Keywords:
Molecular diagnosis;
Nested polymerase chain reaction;
Osteoarticular tuberculosis;
Sensitivity;
Specificity
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(7):581-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To assess the role of polymerase chain reaction (PCR) in serum samples, in the diagnosis of osteoarticular tuberculosis (OTB) in a setting where only clinical and imaging diagnoses determine the treatment. Methods: A total of 44 consecutive serum specimens were collected from clinically suspected OTB patients, based on clinical and radiological [X-ray or magnetic resonance imaging/computed tomography] features. They were screened by in-house nested PCR. In addition, a few specimens were examined by Gram stain, acid-fast bacilli stain, histopathology and routine bacterial culture. A total of 39 specimens were collected from patients suffering from other bone diseases of nontuberculous origin and included as negative controls. Results: Of the 44 clinically suspected OTB patients, in-house nested PCR was positive in 40 (91%) cases; PCR was negative in 38 (97%) negative controls. Sensitivity and specificity of our in-house nested PCR was 90.9% and 97.4%, respectively. The PCR report was available within 48 h. It was possible to standardize serum PCR technique and in positive cases, a good correlation was observed in terms of an adequate treatment response. Conclusions: Nested PCR in serum samples is a rapid, highly sensitive and specific modality for OTB detection. PCR should be performed in addition to clinical evaluation, imaging studies, acid-fast bacilli staining, culture and histopathology diagnosis, if possible.
