Transcellular transport characteristics of huperzine alone or in combination with ginkgolide B across Caco-2 and Madin-Darby canine kidney cell monolayer
10.1016/S1995-7645(14)60155-3
- Author:
Wen-Cui ZHAO
1
;
Qi ZHANG
1
;
Zhi-Hong HOU
2
;
Wei ZHENG
3
Author Information
1. Pharmacy Department, 208th Hospital of PLA.
2. Hospital Office, 208th Hospital of PLA.
3. Infirmary, 208th Hospital of PLA.
- Publication Type:Journal Article
- Keywords:
Caco-2;
Ginkgolide B;
Huperzine A;
Madin-Darby canine renal;
Transcellular Transport
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):889-894
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the various processes involved in transcellular transport (TT) of huperzine A alone or in combination with ginkgolide B in Caco-2 and Madin-Darby canine renal (MDCK) cell monolayer. Methods: The transepithelial passage was assayed in the apical-to-basolateral (AP to BL) direction and opposite direction (BL to AP) in both cell lines. The determination of huperzine A and ginkgolide B were performed by high performance liquid chromatography (HPLC). The passage rates of huperzine A and ginkgolide B were calculated. Bi-directional TT (absorption and secretion) were taken in huperzine A and ginkgolide B in Caco-2 and MDCK cell monolayer. Results: TT absorption and secretion kinetics of huperzine A and ginkgolide B across two cells existed at the same time. The passage rates of huperzine A were increased significantly with adding different concentrations of ginkgolide B. Conclusions: The compound preparations of HA in combination with GB for dementia caused by cerebral ischemic have synergistic effects on the pharmacodynamics, and improve the bioavailability through BBB.
