Immune stimulatory activity of BRP-4, an acidic polysaccharide from an edible plant, Basella rubra L.
10.1016/S1995-7645(14)60148-6
- Author:
Hye-Jin PARK
1
Author Information
1. Department of Food Science and Biotechnology, Gachon University
- Publication Type:Journal Article
- Keywords:
BRP-4;
Immune stimunlatory;
Macrophage;
Phagocytic activity;
Splenocyte proliferation
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):849-853
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluated the immunomodulatory effect of BRP-4, an acidic polysaccharide from Basella rubra (. B. rubra) L on the macrophage activity. Methods: Phagocytic activity was determined by the ingestion of Latex Beads-Rabbit IgG-FITC using the fluorescent microscopy and flow cytometry analysis and nitric oxide production was measured using Griess reaction assay. Results: An enhanced production of NO was observed at 10 and 100 μg/mL of BRP-4. The phagocytic activity of macrophage was enhanced in BRP-4 treated RAW264.7 cells. BRP-4 combined with concanavalin A (Con A) provided obvious promotion and strengthening of the proliferation of the splenocytes. Conclusions: BRP-4, polysaccharide isolated from B. rubra, is suggested to activate macrophage function and stimulate splenocyte proliferation. The strong immunomodulatory activity of BRP-4 confirmed its good potential as an immunotherapeutic adjuvant.
