Preliminary screening for in vitro anti-enteritic properties of a traditional herb Dillenia pentagyna Roxb. fruit extracts
10.1016/S1995-7645(14)60255-8
- Author:
Utpal Chandra DE
1
;
Ranjit GHOSH
1
;
Surajit DEBNATH
2
Author Information
1. Department of Chemistry, Tripura University
2. Department of Medical Laboratory Technology, Women's Polytechnic Hapania
- Publication Type:Journal Article
- Keywords:
Antimicrobial screening;
Basal and selective medium;
Dillenia pentagyna Roxb. fruit extracts;
Municipal sewage microflora Gastrointestinal pathogen;
Urease inhibition assay;
Variable bacterial load
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S332-S341
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore anti-enteric properties of Dillenia pentagyna Roxb. (D. pentagyna) fruit extract fractions of different polarities by comparative antimicrobial activity against municipal sewage microflora and to assess its urease inhibition potential. Methods: Different polar fractions of D. pentagyna fruit extracts were studied by antimicrobial susceptibility test with several adjustments in this resource limited setup. Tests were done against municipal sewage microbes at various bacterial load (initially with 1.0 McFarland followed by 1.5 McFarland) using basal (nutrient agar) and selective medium (MacConkey's agar with and without supplementation of 5% NaCl). All assays statistically scaled with a gradient for uniformity and comparison with ciprofloxacin standard. Fraction with highest activity was studied for urease inhibition potential by kinetic method. Results: DP-47 separated by 30% ethyl acetate (EtOAc) in CHCl
