Expression of transferrin in hematoma brain tissue at different stages after intra cerebral hemorrhage in rats
10.1016/j.apjtm.2015.06.002
- Author:
Long CHEN
1
;
Xue-Gang JIN
1
;
Jian-Fang ZHU
1
;
Jian WANG
1
;
Wen-Hua WANG
1
;
Hui-Juan LI
2
;
Yan-Ping WANG
3
;
You-Xin ZHOU
4
Author Information
1. Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine
2. Department of Endocrinology, Second People's Hospital, Yangzhou University
3. Department of Neurosurgery, Huai'An Hospital, Nanjing Medical University
4. Department of Neurosurgery, First People's Hospital, Suzhou University
- Publication Type:Journal Article
- Keywords:
Intracerebral hemorrhage;
Transferrin;
Transferrin receptor
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(7):574-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the expression of transferrin (Tf) and transferrin receptor (TfR) in hematoma brain tissue at different stage after intracerebral hemorrhage (ICH) in rats. Methods: ICH rats model were established by collagenase method, and rats were sacrificed at 24 h, 72 h, 7 d and 14 d after operation. The levels of Tf and TfR in different periods of rats were detected by immunohistochemical method, and correlation between two groups was analyzed. Results: Tf, TfR-positive cells at each time after operation in observation group were significantly higher than that in control group (P < 0.05). Tf, TfR-positive cells began to increase from 24 h after the operation and reached the peak 72 h-7 d after surgery, but then gradually decreased. Tf was mainly expressed in nucleus and cytoplasm of neurons and glial cells around the hematoma, but TfR was mainly expressed in nucleus and cytoplasm of neurons and choroid plexus endothelial cells. Correlation analysis showed that the Tf-positive cell was significantly positively correlated with TfR-positive cell expression (r = 0.447, P = 0.022). Conclusions: Tf and TfR were important transporters in brain tissue excessive load iron transport after ICH, and detecting the expression levels of the two indicators can provide a reference for prognosis treatment in ICH.
