Causes of immune dysfunction in hyperbilirubinemia model rats
10.1016/S1995-7645(14)60348-5
- Author:
Xiao-Min SUN
1
;
Ping KANG
1
;
Ke TAO
2
Author Information
1. Department of General Internal Medicine, Children's Hospital of Zhengzhou
2. Burn Center of PLA, Xijing Hospital, The Fourth Military Medical University
- Publication Type:Journal Article
- Keywords:
Bilirubin;
Hyperbilirubinemia;
Myd88;
P-p38MAPK;
Spleen cells
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(5):382-385
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the causes of immune dysfunction in neonatal rats with hyperbilirubinemia. Methods: A total of 60 newborn SD rats were equally randomized into normal saline (NS) group, LPS control group, bilirubin control group, low-dose group and high-dose group. After anesthesia, 0.1 mL NS was given to the NS and LPS control group and different doses of bilirubin for the other groups; 1 h later, the NS and bilirubin control group received the intraperitoneal injection of 0.05 mL NS and 1mg/kg LPS for the other groups. After 5 or 24 hours of model establishment, spleens were collected for detecting the expression levels of MyD88 and p-TAK1 protein and the spleen cells apoptosis by immunohistochemmistry and TUNEL method. After 24 hours of model establishment, serum inflammatory factors levels and T cell subsets distribution were determined by ELISA and flow cytometry. Results: In contrast to low-dose bilirubin, high-dose bilirubin could induce spleen cells apoptosis in coordination with LPS. After 5 hours of model establishment, compared with NS group, MyD88 expression level in low-dose group elevated while p-TAK1 level in high-dose group reduced (P<0.05). In high-dose group, inflammotory factors levels and CD8+ T cells percentage were all higher than LPS control and NS group (P<0.05), while CD4+ T cells percentage was lower than NS group (P<0.05). Conclusions: High-concentration plasma bilirubin in coordination with LPS could inhibit NF-κB signal pathways activation and aggravate inflammatory reaction, thus caused immunosuppression with inflammation cascade, which resulted in the immune dysfunction.
