Fruits extracts of Hovenia dulcis Thunb. suppresses lipopolysaccharide-stimulated inflammatory responses through nuclear factor-kappaB pathway in Raw 264.7 cells
10.1016/j.apjtm.2016.03.017
- Author:
Ju-Yeon PARK
1
;
Jin-Young MOON
1
;
Sun-Dong PARK
2
;
Won-Hwan PARK
3
;
Hyuck KIM
3
;
Jai-Eun KIM
4
Author Information
1. Department of Acupoint, College of Korean Medicine, Dongguk University
2. Department of Prescription, College of Korean Medicine, Dongguk University
3. Department of Diagnostics, College of Korean Medicine, Dongguk University
4. Department of Pathology, College of Korean Medicine, Dongguk University
- Publication Type:Journal Article
- Keywords:
Fruits of Hovenia dulcis;
Heme oxygenase-1;
Inflammation;
Macrophage;
Nuclear factor-kappaB
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(4):357-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the anti-inflammatory effects and the action mechanism of the fruits of Hovenia dulcis (H. dulcis) in lipopolysaccharide (LPS)-induced mouse macrophage Raw 264.7 cells. Methods: The extract of H. dulcis fruits (EHDF) were extracted with 70% ethanol. Mouse macrophages were treated with different concentrations of EHDF in the presence and absence of LPS (1 μg/mL). To demonstrate the inflammatory mediators including nitric oxide, inducible nitric oxide synthase and cyclooxygenase (COX)-2 expression levels were analyzed by using in vitro assay systems. COX-derived pro-inflammatory cytokines including interleukin-1β, tumor necrosis factor-α and prostaglandin E
