Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury
10.1016/j.apjtm.2016.05.007
- Author:
Hua FANG
1
;
Jing-Chao ZHANG
1
;
Miao YANG
1
;
Jian-Ping ZHANG
1
;
Fang-Xiang ZHANG
1
;
Hua FANG
2
;
Jing-Chao ZHANG
2
;
Miao YANG
2
;
Jian-Ping ZHANG
2
;
Fang-Xiang ZHANG
2
;
Hua-Feng LI
3
;
Quan-Yun WANG
4
;
Ru-Rong WANG
4
;
Jin LIU
4
Author Information
1. Department of Anesthesiology, Guizhou Provincial People's Hospital
2. Department of Anesthesiology, Affiliated People's Hospital of Guizhou Medical University
3. Department of Anesthesiology, West China Second University Hospital
4. Department of Anesthesiology, West China Hospital, Sichuan University
- Publication Type:Journal Article
- Keywords:
Gastrodin;
Mitochondria;
Motor evoked potential;
Spinal cord ischemia reperfusion injury
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(7):688-693
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of perfusion of the gastrodin in abdominal aorta for alleviating the spinal cord ischemia reperfusion injury (SCIRI). Methods A total of 36 New Zealand white rabbits were divided randomly into sham-operated group (group S), control group (group C) and gastrodin group (group G), 12 rabbits for each group. Aorta abdominalis infrarenalis blocking method was applied to establish the SCIRI model. The changes of motor evoked potentials (MEPs) before the ischemia and on 30 min, 60 min, 6 h, 12 h and 24 h of reperfusion of the gastrodin were respectively recorded, and the neurologic function score before the ischemia, on the 6 h, 12 h and 24 h of the reperfusion of the gastrodin were assessed. And the changes of the concentration of serum neuron specific enolase (NSE), interleukin (IL)-lβ and IL-8 were measured before the ischemia, after 45 min of ischemia, and on 30 min, 60 min, 6 h, 12 h and 24 h of reperfusion of gastrodin. Then the levels of spinal cord nerve cells mitochondrial superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and mitochondrial swelling degree (MSD) were tested and the histopathologic changes in spinal cord tissues were observed. Results The levels of the NSE, IL-lβ, IL-8, ROS, MDA and MSD of group C were all significantly elevated after the ischemia (P < 0.01); the levels of the spinal nerve cell mitochondria SOD, GSH-PX and T-AOC were all significantly reduced (P < 0.01), MEPs and spinal cord tissue pathology were damaged significantly (P < 0.01). The rate of motor neuron abnormalities and the damages of spinal cord tissue pathology of group G were significantly milder than those of group C (P < 0.01); the levels of NSE, IL-lβ, IL-8, ROS, MDA and MSD were significantly lower than those of group C (P < 0.01), but the levels of SOD, GSH-PX and T-AOC were all significantly higher than those of group C (P < 0.01), and the recovery of neurologic function score during the reperfusion of gastrodin was significantly faster than group C (P < 0.01). Conclusions Perfusion of the gastrodin in abdominal aorta can alleviate the spinal cord ischemia reperfusion injury by promoting the mitochondrial antioxidant capacity and inhibiting the inflammatory reaction.
