Optimized combinations of statins and azoles against Acanthamoeba trophozoites and cysts in vitro

Ines SIFAOUI; Carmen MARTÍN-NAVARRO; Atteneri LÓPEZ-ARENCIBIA; María REYES-BATLLE; Basilio VALLADARES; José PINERO; Jacob LORENZO-MORALES; Ines SIFAOUI; Sutherland MACIVER

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Optimized combinations of statins and azoles against Acanthamoeba trophozoites and cysts in vitro

Author: Ines SIFAOUI ¹ ; Carmen MARTÍN-NAVARRO ¹ ; Atteneri LÓPEZ-ARENCIBIA ¹ ; María REYES-BATLLE ¹ ; Basilio VALLADARES ¹ ; José PINERO ¹ ; Jacob LORENZO-MORALES ¹ ; Ines SIFAOUI ² ; Sutherland MACIVER ³
Author Information

1. University Institute of Tropical Diseases and Public Health of the Canary Islands, University of la Laguna
2. Laboratoire Materiaux-Molecules et Applications, IPEST, LA Marsa, University of Carthage
3. Centre for Integrative Physiology, Biomedical Sciences, Edinburgh Medical School, University of Edinburgh
Publication Type:Journal Article
Keywords: Atorvastatin; Fluvastatin; Keratitis; Posaconazole; Simvastatin; Voriconazole
From: Asian Pacific Journal of Tropical Medicine 2019;12(6):283-287
CountryChina
Language:Chinese
Abstract: To evaluate the combination of several statins (atorvastatin, fluvastatin and simvastatin) and azoles (voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the different drug combinations against the trophozoite stage of different Acanthamoeba strains were evaluated by Alamar Blue assay. Effect on the cyst stage was observed by inverted microscope. Cytotoxicity of combinations of azoles and statins was evaluated by measuring the release of lactate dehydrogenase from a murine macrophage cell line. Results: Combinations of any of the tested statins and voriconazole or posaconazole were more efficient in inhibiting Acanthamoeba compared to statins or azoles individually. The drug combinations at the combined inhibitory concentrations 50% showed lower toxicity compared to that of the compounds alone. Conclusions: The combinations of statins together with voriconazole and posaconazole are more efficient than these drugs alone, and these combinations have lower cytotoxicity in mammalian cell lines.