In vitro assessment of the synergism between extracts of Cocos nucifera husk and some standard antibiotics
10.1016/j.apjtb.2016.12.022
- Author:
Taiwo Adesola AKINYELE
1
;
Etinosa Ogbomoede IGBINOSA
1
;
David Ayinde AKINPELU
1
;
Anthony Ifeanyin OKOH
1
;
Taiwo Adesola AKINYELE
2
;
Etinosa Ogbomoede IGBINOSA
2
;
David Ayinde AKINPELU
2
;
Anthony Ifeanyin OKOH
2
Author Information
1. SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare
2. Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare
- Publication Type:Journal Article
- Keywords:
Antibacterial;
Antibiotics;
Indifference;
Inhibition zone;
Synergism;
Time-kill assay
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(4):306-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the interactions between the crude extracts of Cocos nucifera (C. nucifera) and six front line antibiotics (ampicillin sodium salt, penicillin G sodium, amoxicillin, chloramphenicol, ciprofloxacin and tetracycline hydrochloride), against some bacterial pathogens linked with human infection. Methods The pulverized husk of C. nucifera was dissolved in 95% n-hexane and extracted using Soxhlet extraction method and sterile distilled water (aqueous). The antibacterial susceptibility of the crude extracts of C. nucifera was tested against environmental and clinical strains (6) obtained from the South African Bureau of Standards (SABS), Vibrio (6) and Listeria pathogens (6). The agar-well diffusion method was used for screening the extracts for their antibacterial activity. The minimum inhibitory concentration and minimum bactericidal concentration of the extracts were determined. Time-kill assay was used to evaluate bactericidal and/or bacteriostatic activity. The synergistic effect of the crude extracts and antibiotics was assessed and evaluated by adopting the checkerboard methods. Results With the time-kill assay, the highest bactericidal activity was observed on Vibrio fluvialis EL041 with a −5.6 ± 0.2 log
