Sapium ellipticum (Hochst) Pax ethanol leaf extract modulates glucokinase and glucose-6-phosphatase activities in streptozotocin induced diabetic rats
10.1016/j.apjtb.2017.05.009
- Author:
Osasenaga Macdonald IGHODARO
1
;
Oluseyi Adeboye AKINLOYE
1
;
Regina Ngozi UGBAJA
1
;
Osasenaga Macdonald IGHODARO
2
;
Samuel Olatunbosun OMOTAINSE
3
Author Information
1. Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB)
2. Department of Biochemistry, Faculty of Sciences, Lead City University
3. Department of Vertinary Pathology, College of Vertinary Medicine, Federal University of Agriculture, Abeokuta (FUNAAB)
- Publication Type:Journal Article
- Keywords:
Diabetes;
Glucokinase;
Glucose homeostasis;
Glucose-6-phosphatase;
Sapium ellipticum
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(6):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effects of Sapium ellipticum (SE) leaf extract on the hepatic activities of glucokinase and glucose-6-phosphatase in streptozotocin-induced diabetic Wistar rats. Methods STZ-induced diabetic Wistar rats (four groups, n = 8) were used in this study. SE was assessed at two different doses, 400 and 800 mg/kg BW, in comparison with metformin (METF) (12 mg/kg BW) as a reference antidiabetic drug. All treatments were done orally (p.o), twice daily at 8 h interval for a period of 21 days. Glucokinase and glucose-6-phosphatase activities were respectively determined using standard protocols. Hepatic and muscle glycogen contents were estimated as well. Results STZ caused significant decrease in glucose-6-phosphatase activity and concomitant increase in glucokinase activity. SE extract especially at 400 mg dosage significantly reversed the alterations by increasing glucokinase activity by 40.31% and inhibiting glucose-6-phosphatase activity by 37.29% compared to diabetic control animals. However, the effects were significantly lower than that of METF which enhanced glucokinase activity by 94.76% and simultaneously inhibited glucose-6-phosphatase activity by 49.15%. The extract also improved hepatic glycogen level by 32.37 and 27.06% at 400 and 800 mg dosage respectively. HPLC-MS analysis of some SE fractions in dynamic MRM mode (using the optimized compound-specific parameters) revealed among other active compounds, the presence of amentoflavone, which has been associated with antidiabetic function. Conclusions The ability of SE extract to concurrently inhibit glucose-6-phosphatase and activate glucokinase in this study suggests that it may be a treatment option for type 2 diabetes patients, and the presence of amentoflavone in the plant extract may account for its anti-diabetic potential.
