Effect of protease inhibitor from Agaricus bisporus on glucose uptake and oxidative stress in 3T3-L1 adipocytes
- Author:
Reena VISHVAKARMA
1
;
Abha MISHRA
1
Author Information
- Publication Type:Journal Article
- Keywords: 3-diazol-4-yl) amino]-2-deoxy-d-glucose; Oxidative stress; Hydrogen peroxide; 3T3-L1 adipocytes; Protease inhibitor; Agaricus bisporus; 2-[N-(7-nitrobenz-2-oxa-1
- From:Asian Pacific Journal of Tropical Biomedicine 2020;10(3):136-146
- CountryChina
- Language:Chinese
- Abstract: Objective: To explore the effect of the protease inhibitor from Agaricus bisporus (J.E. Lange) Imbach (AbPI) on glucose uptake and oxidative stress in 3T3-L1 adipocytes. Methods: Adipocytes were differentiated and stained with Oil-Red-O staining to confirm adipogenesis. The toxic/protective effect of AbPI on the adipocytes was determined by MTT assay, intracellular reactive oxygen species generation through flow cytometry, and morphologically through confocal microscopy using propidium iodide, 4,6-diamino-2-phenylindol dihydrochloride, and 2',7'-dichlorofluorescein diacetate dyes. The uptake of fluorescent glucose analog, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose by adipocytes was also studied through confocal microscopy. Results: MTT assay showed that the cell survival rate was (28.00±3.00)%, (92.33±2.60)%, and (71.34±2.10)% in the presence of 2 mM H
