Antidiabetic effect of Chrysophyllum albidum is mediated by enzyme inhibition and enhancement of glucose uptake via 3T3-L1 adipocytes and C2C12 myotubes

Benjamin HARLEY; Theophilus FLEISCHER; Benjamin HARLEY; Rita DICKSON; Isaac AMPONSAH; Robert NGALA; Dorice BERKOH; Dorice BERKOH

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Antidiabetic effect of Chrysophyllum albidum is mediated by enzyme inhibition and enhancement of glucose uptake via 3T3-L1 adipocytes and C2C12 myotubes

Author: Benjamin HARLEY ¹ ; Theophilus FLEISCHER ¹ ; Benjamin HARLEY ² ; Rita DICKSON ² ; Isaac AMPONSAH ² ; Robert NGALA ³ ; Dorice BERKOH ³ ; Dorice BERKOH ⁴
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1. Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, University of Health and Allied Sciences
2. Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology
3. Department of Molecular Medicine, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology
4. Department of Basic and Applied Biology, School of Sciences, University of Energy and Natural Resources
Publication Type:Journal Article
Keywords: Antidiabetic; C2C12 myotubes; Chrysophyllum albidum; Glucose uptake; α-amylase inhibition; α-glucosidase inhibition
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(9):387-396
CountryChina
Language:Chinese
Abstract: Objective: To investigate the in vivo and in vitro antidiabetic potential of Chrysophyllum albidum. Methods: The effects of oral treatment with hydro-ethanolic extract (125, 250 and 500 mg/kg) of the stem bark of Chrysophyllum albidum and glibenclamide for 21 d on glucose level, serum enzyme markers for liver function, lipid profile, total protein, serum urea, serum creatinine, and body weight were evaluated in experimental diabetic rats administered with 45 mg/kg of streptozotocin. In vitro assays including glucose uptake in C2C12 cells and 3T3-L1 adipose tissues, α-glucosidase and α-amylase inhibition were employed to evaluate the possible mechanism of hypoglycemic action of the extract. DPPH and nitric oxide radical antioxidant activity of the extract was also measured. Results: The increased levels of blood glucose, triglycerides, low-density lipoprotein, total cholesterol, serum aspartate, and alanine transaminases, creatinine, and urea in the diabetic animals were reduced significantly (P<0.01) after treatment with Chrysophyllum albidum extract. The decreased total protein and high-density lipoprotein concentrations were normalized after treatment. In addition, the extract significantly (P<0.01) increased the transport of glucose in 3T3-L1 cells and C2C12 myotubes and exhibited considerable potential to inhibit α-amylase and α-glucosidase. It also demonstrated potent antioxidant action by scavenging considerably DPPH and nitric oxide radicals. Conclusions: Chrysophyllum albidum stem bark extract exhibits considerable antidiabetic effect by stimulating glucose uptake and utilization in C2C12 myotubes and 3T3-L1 adipocytes as well as inhibiting the activities of α-amylase and α-glucosidase.