In vitro anti-melanoma effect of polyphenolic compounds

Fairouz SIOUD; Mouna MAATOUK; Imen Mokdad BZEOUICH; Leila Chekir GHEDIRA; Soumaya KILANI-JAZIRI; Soumaya KILANI-JAZIRI; Imen Mokdad BZEOUICH

Return

In vitro anti-melanoma effect of polyphenolic compounds

Author: Fairouz SIOUD ¹ ; Mouna MAATOUK ¹ ; Imen Mokdad BZEOUICH ¹ ; Leila Chekir GHEDIRA ¹ ; Soumaya KILANI-JAZIRI ¹ ; Soumaya KILANI-JAZIRI ² ; Imen Mokdad BZEOUICH ³
Author Information

1. Unit of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dentistry University of Monastir
2. Department of Pharmaceutical Sciences A, Faculty of Pharmacy of Monastir University of Monastir
3. Faculty of Medecine of Monastir University of Monastir
Publication Type:Journal Article
Keywords: Cell cycle; Cytotoxic activity; Melanin content; Melanoma; Polyphenols; Tyrosinase activity
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(10):446-452
CountryChina
Language:English
Abstract: Objective: To evaluate the effects of phenolic acids (caffeic, ferulic, and coumaric acids) and flavones (luteolin and apigenin) on the proliferation and melanogenesis in murine melanoma B16-F10 cells. Methods: Cell proliferation was determined after 24 and 48 hours of incubation using MTT assay. The effects of these tested compounds on cell cycle progression were analyzed by flow cytometry. Moreover, the melanin content and tyrosinase activity were measured spectrophotometrically at 475 nm. Results: Luteolin and apigenin exhibited significant anti-proliferative activity against B16-F10 cells, while caffeic, ferulic, and coumaric acids induced slight inhibition after 24 and 48 hours of incubation. The tested compounds disturbed cell cycle progression of B16-F10, by a subsequent decrease in G