Erianin inhibits oral cancer cell growth, migration, and invasion via the Nrf2/HO-1/ GPX4 pathway

Author: Xiu-Yun TIAN ¹ ; Rui HAN ¹ ; Mei-Yun ZHOU ¹ ; Bin LUO ¹ ; Xin-Ru CHEN ¹ ; Jin-Cheng XU ¹ ; Qing-Yang HUANG ²
Author Information

1. Department of Stomatology, the First Affiliated Hospital of Bengbu Medical College
2. Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals
Publication Type:Journal Article
Keywords: Erianin; Ferroptosis; Nrf2/HO-1/GPX4; Oral cancer; Reactive oxygen species; Therapy
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(10):437-445
CountryChina
Language:English
Abstract: Objective: To evaluate the effect of erianin on the viability, migration, and invasion of KB cells and elucidate its underlying mechanisms. Methods: Cell Counting Kit-8, colony formation, wound healing, and Transwell assays were used to determine the proliferation, migration, and invasion of oral cancer KB cells. Furthermore, malondialdehyde (MDA) and glutathione (GSH) levels were determined. Fluorescent probes were used to detect changes in intracellular reactive oxygen species and iron ions. Additionally, the expressions of ferroptosis-related proteins, NF-E2-related factor 2 (Nrf2), ferritin heavy chain 1 (FTH1), heme oxygenase 1 (HO-1), and glutathione peroxidase 4 (GPX4) were analyzed by Western blotting assays. Results: Erianin induced ferroptosis and inhibited the proliferation, migration, and invasion of KB cells. Moreover, erianin decreased GSH level, increased MDA level, elevated intracellular ROS and Fe