Detection Value of EB Virus DNA, IL-2, and IL-6 Level in Peripheral Blood of Children with Infectious Mononucleosis.
10.19746/j.cnki.issn.1009-2137.2022.04.046
- Author:
Qiang ZHANG
1
;
Qing-Feng FANG
1
;
Zhi YANG
1
;
Bi-Quan CHEN
2
,
3
Author Information
1. Department of Infectious Diseases, Anhui Provincial Children's Hospital, Hefei 230051, Anhui Province, China.
2. Department of Infectious Diseases, Anhui Provincial Children's Hospital, Hefei 230051, Anhui Province, China,E-mail: asa2253@
3. com.
- Publication Type:Journal Article
- Keywords:
DNA;
EB virus;
infectious mononucleosis;
interleukin-2;
interleukin-6
- MeSH:
Child;
DNA, Viral;
Epstein-Barr Virus Infections;
Hepatomegaly;
Herpesvirus 4, Human;
Humans;
Infectious Mononucleosis/diagnosis*;
Interleukin-2/blood*;
Interleukin-6/blood*;
Splenomegaly
- From:
Journal of Experimental Hematology
2022;30(4):1262-1266
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigative the detection value of EB virus DNA (EBV-DNA), interleukin-2 (IL-2), and interleukin-6 (IL-6) level in peripheral blood of children with infectious mononucleosis (IM).
METHODS:A total of 59 children clinically confirmed with IM in Anhui Provincial Children's Hospital from January 2018 to September 2020 were enrolled as IM group, while other 30 healthy children undergoing physical examination during the same period were enrolled as healthy group. The level of EBV-DNA load, IL-2, and IL-6 were compared between the two groups, and their diagnostic values for IM children were explored. According to the median level of EBV-DNA load, positive children were divided into high viral load group and low viral load group. The hepatomegaly and splenomegaly, and levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), IL-2, and IL-6 were compared between the two groups. The relationship between EBV-DNA load and IL-2, IL-6 levels were explored.
RESULTS:The positive rate of EBV-DNA was 67.80% in IM group, which was significantly higher than 10.00% in healthy group (P<0.001), and the levels of serum IL-2 and IL-6 were also significantly higher than healthy group (P<0.001). The results of ROC curve analysis showed that AUC of IL-2 combined with IL-6 and EBV-DNA load was 0.948, which was significantly greater than that of IL-2, IL-6, and EBV-DNA load alone (0.847, 0.728, 0.789) (P<0.001). The cut-off value of IL-2 and IL-6 was 15.545 pg/ml and 56.560 pg/ml, respectively. Both the proportions of cases with moderate to severe hepatomegaly and splenomegaly in high viral load group were significantly higher than those in low viral load group (P<0.01, P<0.05). The levels of ALT, AST, and IL-2 in high viral load group were significantly higher than those in low viral load group (P<0.001), as well as IL-6 (P<0.01). In high and low viral load groups, EBV-DNA load was positively correlated with IL-2 and IL-6 (in high viral load group, rIL-2=0.598, rIL-6=0.416; in low viral load group, rIL-2=0.621, rIL-6=0.527, P<0.001).
CONCLUSION:The detection of EBV-DNA load combined with IL-2 and IL-6 can improve the diagnostic accuracy of IM, and EBV-DNA load, IL-2 and IL-6 levels are related to the disease progression.
