Irritant toxicity and lectin content of different processed products of Pinelliae Rhizoma.
10.19540/j.cnki.cjcmm.20211207.301
- Author:
Yan-Qiu CHENG
1
;
Hong-Li YU
2
;
Hao WU
2
;
Xing-Bao TAO
1
;
Yu-Wei XIE
1
;
Sheng-Jun CHEN
3
;
Ping ZHANG
4
;
Song LI
3
;
Cai-Xia WANG
1
;
He-Peng WANG
1
;
Ping ZENG
1
;
Bing-Bing LIU
1
Author Information
1. Pharmacology College, Nanjing University of Chinese Medicine Nanjing 210023,China.
2. Pharmacology College, Nanjing University of Chinese Medicine Nanjing 210023,China Jiangsu Key Laboratory of Chinese Medicine Processing Nanjing 210023,China Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing Nanjing 210023,China.
3. Jiangyin Tianjiang Pharmaceutical Co.,Ltd. Jiangyin 214400,China.
4. National Institutes for Food and Drug Control Beijing 100050,China.
- Publication Type:Journal Article
- Keywords:
Pinelliae Rhizoma;
Western blotting for lectin;
irritant toxicity;
processing
- MeSH:
Animals;
Drugs, Chinese Herbal/toxicity*;
Irritants;
Lectins;
Pinellia;
Rabbits;
Technology, Pharmaceutical/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(17):4627-4633
- CountryChina
- Language:Chinese
-
Abstract:
The present study aims to investigate the correlation between irritant toxicity variation and lectin content variation during the processing of Pinelliae Rhizoma products and to explore the feasibility of Western blot as a method for the detection of lectin. We processed Pinelliae Rhizoma Praeparatum Cum Alumine, Pinelliae Rhizoma Praeparatum, and Pinelliae Rhizoma Praeparatumcum Zingibere et Alumine to different degrees and then analyzed their irritant toxicity via Draize rabbit eye test. Western blot was employed to determine the lectin content in Pinelliae Rhizoma products processed with different methods. The correlation between toxicity variation and lectin content variation was then analyzed. Different decoction pieces of Pinelliae Rhizoma were collected for the determination of lectin content. The three processed products of Pinelliae Rhizoma showed gradually decreased toxicity and lectin content as the processing continued. The decreasing trend of lectin content was consistent with that of irritant toxicity during processing, which indicated that the change in lectin content could reflect the trend of irritant toxicity. No band of lectin appeared in the Western blot of processed products of Pinelliae Rhizoma, which suggested that western blotting can be used for the detection of toxic lectin in the processed products of Pinelliae Rhizoma. Lectin should not be detected in the Pinelliae Rhizoma products processed according to the methods in the Chinese Pharmacopoeia.
