Progress of target determination and mechanism of bioactive components of traditional Chinese medicine.
10.19540/j.cnki.cjcmm.20220330.201
- Author:
Xue-Fen WU
1
;
Xiao-Hong WEI
1
;
Yu-Zhuo WU
1
;
Gui-Yang XIA
1
;
Huan XIA
1
;
Ling-Yan WANG
1
;
Hong-Cai SHANG
1
;
Sheng LIN
1
Author Information
1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital,Beijing University of Chinese Medicine Beijing 100700, China.
- Publication Type:Review
- Keywords:
bioactive components of traditional Chinese medicine;
biolayer interferometry;
fluorescence resonance energy transfer;
molecular docking;
protein crystallization;
proteome chip;
surface plasma resonance;
target fishing;
target mutant
- MeSH:
Drugs, Chinese Herbal/pharmacology*;
Medicine, Chinese Traditional;
Molecular Docking Simulation;
Proteome
- From:
China Journal of Chinese Materia Medica
2022;47(17):4565-4573
- CountryChina
- Language:Chinese
-
Abstract:
The pharmacodynamic substances of traditional Chinese medicine(TCM) are the basis for the research of TCM and the development of innovative drugs. However, the lack of clarity of targets and molecular mechanisms is the bottleneck problem that restricts the research of pharmacodynamic substances of TCM. Bioactive components are the material basis of the efficacy of TCM, which exert activity by regulating the corresponding targets. Therefore, it is very important to identify the targets of the bioactive components to elucidate the pharmacological mechanism of TCM. Proteins are the most important drug targets, and study of the interaction between the proteins and bioactive components of TCM plays a key role in the development of pharmacological mechanism of TCM. In recent years, the main techniques for detecting the interaction between the bioactive components and proteins include surface plasmon resonance, fluorescence resonance energy transfer, bio-layer interference, molecular docking, proteome chip, target fishing, target mutant, and protein crystallization techniques, etc. This review summarized the biological target detection techniques and their applications in locating the targets of the bioactive components in TCM in the last decade, and this paper will provide useful strategies to elucidate the pharmacological mechanisms of TCM.
